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目的探讨β-榄香烯介入治疗对兔VX2肾移植癌的作用及其机制。方法建立兔VX2肾移植癌模型,将动物随机分为生理盐水对照组,碘油栓塞,丝裂霉素、5-氟尿嘧啶、β-榄香烯、顺铂、卡铂、阿霉素、噻替哌、环磷酰胺和长春新碱等药物介入治疗组。各组予以相应干预措施,采用螺旋CT及B超动态监测各组肿瘤体积变化并计算平均抑瘤率。治疗后1、7、14d作血液生化检查。治疗后14d采用光镜及透射电镜观察肿瘤组织的形态变化,免疫组化法检测Bax和Bcl-2的表达。结果不同药物介入治疗对兔VX2肾移植癌的效果差异有统计学意义。兔VX2肾移植癌对碘油栓塞、丝裂霉素、顺铂、卡铂高度敏感,肾功能损伤严重;对β-榄香烯、阿霉素、5-氟尿嘧啶中度敏感,β-榄香烯对肾功能损伤较轻微;对噻替哌、环磷酰胺、长春新碱耐药。光镜及透射电镜下β-榄香烯治疗组以肿瘤细胞凋亡为主,偶见细胞自溶;Bax表达显著上调(P<0.05),Bcl-2表达差异无统计学意义(P>0.05)。结论β-榄香烯介入治疗肾癌效果显著,肾脏损伤轻微,其作用机制以促进肿瘤细胞凋亡为主,Bax参与该途径。
Objective To investigate the effect of β-elemene interventional therapy on VX2 renal carcinoma in rabbits and its mechanism. Methods Rabbit VX2 kidney transplantation model was established. The animals were randomly divided into normal saline control group, lipiodol embolization, mitomycin, 5-fluorouracil, β-elemene, cisplatin, carboplatin, doxorubicin, Piperazine, cyclophosphamide and vincristine and other drug intervention group. The interventions were given to each group. The volume of tumor in each group was dynamically monitored by spiral CT and B-mode ultrasonography, and the average tumor inhibition rate was calculated. Blood biochemical tests were performed on days 1, 7 and 14 after treatment. The morphological changes of tumor tissues were observed by light microscope and transmission electron microscope 14 days after the treatment, and the expressions of Bax and Bcl-2 were detected by immunohistochemistry. Results The results of different drug interventions on rabbit VX2 renal allograft cancer were significantly different. Rabbit VX2 renal transplanted carcinoma of lipiodol embolism, mitomycin, cisplatin, carboplatin highly sensitive, severe renal damage; β-elemene, doxorubicin, 5-fluorouracil moderate sensitivity, β- Ethylene damage to renal function is minor; to thiotepa, cyclophosphamide, vincristine resistance. The apoptosis of tumor cells was predominant in β-elemene treated group under light and transmission electron microscopy, with occasional cell autolysis; the expression of Bax was significantly up-regulated (P <0.05), while the expression of Bcl-2 was not significantly different (P> 0.05 ). Conclusion β-elemene interventional treatment of renal cell carcinoma has a significant effect, minor renal damage, its mechanism of action to promote tumor cell apoptosis, Bax involved in this pathway.