按Langendorff技术灌注鼠心脏,观察L-精氨酸对缺血-再灌注诱导的一氧化氮合酶(NOS)组织活性变化和心肌功能障碍的影响。 方法:将大鼠分为非缺血性心脏灌注及完全缺血30min后再灌注30min两类,缺血灌注类大鼠在缺血开始时分别用L-精氨酸(1 mmol/L),D-精氨酸(1 mmol/L),NOS抑制剂L-NNA(1 mmol/) Rats were perfused with Langendorff technique to observe the effect of L-arginine on changes of tissue nitric oxide synthase (NOS) activity and myocardial dysfunction induced by ischemia-reperfusion. Methods: The rats were divided into two groups: non-ischemic cardiac perfusion and reperfusion 30 min after complete ischemia, ischemia-reperfusion rats were induced with L-arginine (1 mmol / L) D-arginine (1 mmol / L), NOS inhibitor L-NNA (1 mmol / L)