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以ｐＵＣ１９－δｇｌｏｂｉｎ为模板，用ＰＣＲ定点突变技术将δ珠蛋白基因启动子区－６４位的Ｃ突变为Ｔ，即将ＣＣＡＡＣ盒改变为ＣＣＡＡＴ，并获得野生及突变的δ珠蛋白基因启动子区约３００ｂｐ的两个片段。将此两片段分别克隆到以虫荧光素酶为报告基因的表达载体ＰＭＧ３中，转染人ＨｅＬａ细胞，以瞬时表达分析突变的ＣＣＡＡＣ盒的启动子功能。结果表明突变组发动荧光素酶表达而产生的发光强度为野生型的５倍，说明将δ珠蛋白基因启动子区－６４位的ＣＣＡＡＣ盒改变为ＣＣＡＡＴ能够增强该启动子的功能，从而证实了δ基因启动子区ＣＣＡＡＣ盒的结构特点是该基因表达水平低的主要原因之一。
Using pUC19-δglobin as a template, the C-globin gene promoter-C at position 64 was mutated to T by PCR site-directed mutagenesis. The CCAAC box was changed to CCAAT and the wild-type and mutant δ-globin gene promoter regions were obtained Two fragments of 300bp. The two fragments were respectively cloned into the expression vector PMG3 with luciferase reporter gene and transfected into human HeLa cells to analyze the promoter function of the mutant CCAAC box by transient expression. The results showed that the luciferase expression in the mutant group produced a luminous intensity of 5 times that of the wild type, indicating that changing the CCAAC box at position 64 of the globin gene promoter into CCAAT can enhance the function of the promoter, The structural characteristics of the CCAAC box in the delta gene promoter region is one of the major reasons for the low expression level of this gene.