用免疫组织化学双重染色法,在一张切片上同时显示胰岛PP细胞与A细胞。观察生后发育期间大鼠全胰PP细胞、A细胞和PP-Glu共存细胞的形态、分布和数量。结果表明,PP细胞突起可伸达A细胞,PP和Glu在胞质内共存呈分散或分区两种形式,胰岛可分为PP细胞型、A细胞型和PP-A细胞型,PP细胞和PP-Glu共存细胞的计数表明,在生后7天最低,28天最高。 Immunohistochemical double staining showed that islet PP cells and A cells were displayed simultaneously on one section. The morphology, distribution and quantity of PP-A, PP-Glu and co-existent cells in whole rat pancreas during postnatal development were observed. The results showed that PP cells can reach A cell protrusion, PP and Glu coexist in the cytoplasm was dispersed or divided into two forms, islet can be divided into PP cell type, A cell type and PP-A cell type, PP cells and PP The counts of -Glu coexisting cells showed the lowest at 7 days after birth and the highest at 28 days.