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为研究不同给药方式下,恩诺沙星在中华草龟体内的药物代谢动力学规律。选取20只健康中华草龟,每组10只,随机分为2组,分别为肌注组和胃插管强制口服组,给药量均为10 mg/kg,应用液相色谱串联质谱法测定数据,Kinetic 4.4软件的非房室模型分析药时数据。胃插管强制口服给药主要药动学参数为C_(max)7.49μg/mL、T_(max) 12 h、T_(1/2)λz为99.85 h、AUC_(0-∞)为531.67 h~*μg/mL;肌注给药主要药动学参数为C_(max)5.85μg/mL、T_(max) 4 h、T_(1/2)λz为30.42 h、AUC_(0-∞)为193.6 h~*μg/mL。2种不同给药条件下,肌注恩诺沙星比胃插管强制口服更快达到最高血药浓度,表明肌注恩诺沙星在中华草龟体内吸收更快;胃插管强制口服给药的C_(max)和AUC均高于比肌注给药,表明胃插管强制口服恩诺沙星在中华草龟体内吸收更完全,分布更广泛效果更好。恩诺沙星在中华草龟体内消除缓慢,MRT长,胃插管强制口服给药MRT显著长于肌注给药,其血药浓度保留时间长,效果更持久。研究表明,需较快达到疗效时,建议肌注恩诺沙星;需持续给药且龟体代谢状态正常时,建议胃插管强制口服恩诺沙星;恩诺沙星在胃插管强制口服给药条件下,在中华草龟体内血药浓度高,保留时间长,生物利用度高,更适宜作为中华草龟个体疾病治疗的给药方式。
To study the pharmacokinetics of enrofloxacin in Chinese turtles under different administration modes. Twenty healthy Chinese tortoises (TCCs) were selected and randomly divided into two groups, namely intramuscular injection group and gastric intubation-forced oral group. The dosage was 10 mg / kg, which was determined by liquid chromatography-tandem mass spectrometry Data, Kinetic 4.4 software for non-compartmental analysis of drug data. The main pharmacokinetic parameters of forced oral administration of gastric intubation were Cmax 7.49μg / mL, T max 12h, T 1/2 λz 99.85 h, and AUC 0- ∞ 531.67 h ~ * μg / mL. The main pharmacokinetic parameters of intramuscular injection were Cmax 5.85μg / mL, T max 4 h, T 1/2 λz 30.42 h, and AUC 0-∞ 193.6 h ~ * μg / mL. Under the two different administration conditions, enrofloxacin intramuscular injection forced to reach the highest plasma concentration faster than gastric intubation, indicating that intramuscular injection of enrofloxacin absorbed faster in Chinese tortoises; gastric intubation forced oral administration The C_ (max) and AUC of the drug were higher than those of the intramuscular injection, which indicated that gastric intubation forced oral enrofloxacin to absorb more completely and distribute more widely in Chinese tortoise body. Enrofloxacin slowly eliminated in Chinese tortoise, MRT length, gastric intubation mandatory oral administration of MRT was significantly longer than intramuscular administration, the plasma concentration retention time is longer, the effect is more lasting. Studies have shown that the need to achieve faster efficacy, the proposed intramuscular injection of enrofloxacin; continuous administration and turtle metabolic status is normal, it is recommended gastric intubation forced oral enrofloxacin; enrofloxacin in the gastric intubation mandatory Oral administration, the Chinese tortoise body blood concentration, long retention time, high bioavailability, more suitable for Chinese turtles individual disease treatment delivery.