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目的:观察补肝健腰方对腰椎间盘退变大鼠椎间盘TNF-α(Tumor Necrosis Factor-α)及NF-κB(Nuclear Factor-Kappa B)表达的影响。方法:SD大鼠75只,随机分为假手术组、模型组、腰痹通组、芬必得组、补肝健腰组,每组各15只,成功制备大鼠腰椎间盘退变模型,分别给予腰痹通胶囊、芬必得、补肝健腰方药物灌胃,检测各组干预前、干预第20天和第40天时椎间盘TNF-α及NF-κB表达,进行对比。结果:干预第20天和第40天,腰痹通组、芬必得组、补肝健腰组椎间盘TNF-α及NF-κB表达较模型组下降,差异有统计学意义(P<0.05),腰痹通组、芬必得组、补肝健腰组在干预第20天和第40天时椎间盘TNF-α及NF-κB表达水平,分别与本组干预前比较,差异有统计学意义(P<0.05),补肝健腰组在以上各时间点与腰痹通组、芬必得组比较,差异无统计学意义(P>0.05)。结论:补肝健腰方能降低腰椎间盘退变模型大鼠椎间盘TNF-α及NF-κB表达,这可能为该方可以防治椎间盘退变疾病的作用机理。
Objective: To observe the effects of Bugan Jianhou Formula on the expression of tumor necrosis factor-α (TNF-α) and nuclear factor-kappa B (NF-κB) in rats with lumbar disc degeneration. Methods: Seventy-five SD rats were randomly divided into sham-operation group, model group, Yaobitong group, Fenbid group and Bugan Jianlv group, 15 rats in each group. Rats with lumbar disc degeneration The rats in each group were given gavage capsule and Fenbi Decoction and Bugan Jianliao Decoction. The levels of TNF-αand NF-κB in intervertebral disc were measured before and 20 and 40 days after intervention. Results: The expression of TNF-α and NF-κB in the Lumbiont group, Fenbid group, Buganjianlian group on the 20th day and the 40th day were significantly lower than those in the model group (P <0.05) The levels of TNF-α and NF-κB in intervertebral discs on the 1st, 2nd, and 40th days in the intervention groups were significantly higher than those in the control group (P < 0.05). There was no significant difference between Buganjiangyuan group and Yaobitong group and Fenbid group at all the above time points (P> 0.05). Conclusion: Bugan Jianlou Decoction can reduce the expression of TNF-α and NF-κB in the intervertebral disc of lumbar disc degeneration rats, which may be the mechanism of the prevention and treatment of degenerative disc disease.