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Objective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibroblasts.Methods Rat lung fibroblasts and alveolar macrophages were cultured.A transdifferentiation model of rat lung fibroblasts was established.Free silica was used as a stimulator for rat lung fibroblasts.Changes in α-SMA were detected by immunohistochemistry and Western blot,respectively.Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis(2-DE).Results Six protein spots were identified by mass spectrometry,including glyceraldehyde 3-phosphate-dehydrogenase,peroxiredoxin 5,heterogeneous nuclear ribonucleoprotein A2,transgelin 2,keratin K6 and vimentin.Conclusion Some proteins are changed in free silica-induced transdifferentiaed rat lung fibroblasts.
Objective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibroblasts. Methods Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in α-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis (2-DE). Results Six protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate- dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin.Conclusion Some proteins are changed in free silica-induced transdifferentiaed rat lung fibroblasts.