弓形虫是一种单细胞寄生性原虫,只能在活的有核细胞内生长繁殖,而不能在人工合成的培养基上生长,也不能冻干保存。因此,弓形虫通常采用小鼠腹腔接种传代的方法保存,目前国内报道保存方法较复杂,为寻找一种简单易行的方法,根据文献的报道,作者对两种方法进行比较,并在方法学上作了一些改进,为深入开展弓形虫的分子生物学实验研究提供了物质基础。1材料与方法1.1弓形虫:RH珠由天津医学院寄生虫教研室提供。 Toxoplasma gondii is a unicellular parasitic protozoan, can only grow and multiply in living nucleated cells, but can not grow on artificial synthetic medium, nor freeze-dried. Therefore, Toxoplasma gondii is usually preserved by the method of abdominal passage inoculation in mice. At present, the methods of preservation in domestic reports are complex. In order to find a simple and easy method, according to the reports in the literature, the authors compare the two methods, Made some improvements, and provided the material basis for further study on the molecular biology of Toxoplasma gondii. 1 Materials and Methods 1.1 Toxoplasma gondii RH beads from the Tianjin Medical College parasite provided.