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目的 研究传染性非典型肺炎又称严重急性呼吸综合征(SARS),探讨患者在不同发病阶段血清中多种细胞因子的变化、发病机理及与免疫反应的关系。方法 应用Randox公司产生的EVIDENCE180全自动化学发光生物芯片分析仪,对90名健康成年人和34例确诊SARS患者的早、中、晚3期血液标本分别进行了12项细胞因子的定量检测,以比较不同发病阶段各种细胞因子的变化趋势。结果 SARS患者血清中的细胞因子水平除IL-1β没有显著变化外,其他各项变化均比较明显。其中,IL-6、IL-8、IL-10及干扰素γ与健康人比较显著上升;而IL-1α、IL-2、IL-4、血管内皮生长因子、表皮生长因子、单核细胞趋化蛋白-1及肿瘤坏死因子α与健康对照组比较显著下降。结论 各种血清细胞因子在SARS患者不同发病时期有较大变化,发病早期尤为明显,本结果进一步支持SARS患者的病理损伤过程与免疫失调有关。
Objective To study SARS, also known as Severe Acute Respiratory Syndrome (SARS), and to explore the changes of serum cytokines, pathogenesis and immune response in patients with different stages of SARS. Methods Using the EVIDENCE180 automated chemiluminescence biochip analyzer produced by Randox Corporation, 12 cytokines were detected in 90 healthy adults and 34 SARS patients with early, Compare the trend of different cytokines in different stages of disease. Results Serum levels of cytokines in SARS patients showed no obvious changes except IL-1β. Among them, IL-6, IL-8, IL-10 and IFN-γ were significantly increased compared with those in healthy subjects. IL-1α, IL-2, IL-4, VEGF, EGF, Compared with healthy control group, protein-1 and tumor necrosis factor-α decreased significantly. Conclusion All kinds of serum cytokines have great changes in different stages of onset of SARS patients, especially in the early stage of the disease. The results further support the pathogenesis of SARS patients with pathological damage and immune disorders.