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应用ＲＴ－ＰＣＲ和免疫组化检测大肠粘膜、腺瘤及腺癌组织ＤＣＣｍＲＮＡ和ＰＣＮＡ表达状况。结果表明：（１）大肠粘膜中均能检出ＤＣＣｍＲＮＡ表达，９例腺瘤１例ＤＣＣｍＲＮＡ表达缺失（１１．１％），３３例腺癌２０例ＤＣＣｍ－ＲＮＡ表达缺失（６０．６％）。（２）大肠癌中ＤＣＣｍＲＮＡ表达缺失与分化程度、浸润深度及转移有显著相关性（Ｐ＜０．０１）。（３）大肠癌ＤＣＣｍＲＮＡ表达缺失与ＰＣＮＡ指数显著相关（Ｐ＜０．００１）。提示：检测大肠癌ＤＣＣｍＲＮＡ表达有助于判断大肠癌的恶性表型。其可能作用机制是ＤＣＣ基因失活后导致其抑制细胞增殖的特性丧失，从而使大肠癌细胞获得更强的浸润和转移能力。
RT-PCR and immunohistochemistry were used to detect DCC mRNA and PCNA expression in colorectal mucosa, adenoma and adenocarcinoma. The results showed that: (1) DCC mRNA expression was detected in the large intestine mucosa, DCCmRNA expression was absent in 11 cases of adenoma (11.1%), and DCCm-RNA expression was absent in 20 cases of adenocarcinoma (60.6%). (2) DCC mRNA expression in colorectal cancer was significantly correlated with differentiation, depth of invasion, and metastasis (P<0.01). (3) The loss of DCC mRNA expression in colorectal cancer was significantly associated with PCNA index (P<0.001). Tip: Detection of DCC mRNA expression in colorectal cancer can help to determine the malignant phenotype of colorectal cancer. Its possible mechanism is that the inactivation of the DCC gene leads to its loss of cell proliferation, resulting in a greater ability to infiltrate and metastasize colorectal cancer cells.