论文部分内容阅读
聚乙烯吡咯酮碘(PVP碘)是一水溶性局部制菌剂,作者用以灌洗腹腔,观察其对腹膜防御机制的影响,包括腹腔液中细胞的类型、杀菌活力、吞噬功能 以及多形核白细胞(PmNL)的趋化性.取90只Wis-tar白鼠,各重160～200g,等分成3组:第Ⅰ组测定PVP碘对腹腔液的杀菌活力,再分成3小组,每小组10只鼠.IA组—在全身麻醉下剖腹,用1%PVP碘20ml灌洗,关腹.4小时后取腹腔液,按Bergman法测定其杀菌活力;ⅠB组—按同样方法剖腹和测定,但不用PVP碘灌洗;ⅠC组—方法同ⅠA组,但在剖腹前和剖腹后即刻测腹腔液杀菌活力.第Ⅱ组 测定PVP碘对腹膜PMNL趋化性的影响,等分成3小组.ⅡA—取腹腔液前24小时,自心脏穿刺采血2.5ml,取其血清以灭活补体.ⅡA组—同ⅠA
Povidone (PVP) is a water-soluble topical bacteriostat that the authors used to lavage the peritoneal cavity to observe its effects on peritoneal defense mechanisms including the type of cell in the peritoneal fluid, bactericidal activity, phagocytosis, and polymorphism The chemotaxis of PmNL was determined.Among 90 Wis-tar rats weighing 160 ~ 200g, were equally divided into 3 groups: group I was used to determine the bactericidal activity of PVP iodine on peritoneal fluid, and then divided into 3 groups of 10 Group IA - laparotomy under general anesthesia, lavage with 1% PVP iodine 20ml, abdominal closure. After 4 hours, the peritoneal fluid was taken and the bactericidal activity was determined by the method of Bergman; Group IB - laparotomy and determination by the same method Group ⅠC - method with Ⅰ A group, but before cesarean section and immediately after celiotomy measured peritoneal bactericidal activity.Phase Ⅱ measured PVP iodine PMNL peritoneal chemotaxis, equally divided into three groups.ⅡA- Take 24 hours before the intraperitoneal fluid, blood from the heart puncture 2.5ml, take its serum to inactivate complement. Ⅱ A group - with Ⅰ A