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目的研究野鸦椿Euscaphis japonica根抑制肝脂堆积活性部位及其化学成分。方法萃取法制备不同极性部位,以油酸诱导的Hep G2细胞脂堆积模型、油红O染色观察细胞内脂滴的堆积,并检测细胞内三酰甘油(TG)的量筛选抑制肝脂堆积活性部位,并采用硅胶、Sephadex LH-20、HPLC等多种色谱技术对活性部位进行分离纯化,根据理化性质和波谱数据鉴定化合物结构。结果石油醚部位能显著抑制油酸诱导的Hep G2细胞内脂滴的堆积并降低TG的量,从中分离得到9个化合物,分别鉴定为3β,19-二羟基-24-反式-阿魏酰基-熊果烷-12-烯-28-酸(1)、β-谷甾醇(2)、7-羟基-2-辛烯-5-内酯(3)、3,3′-二甲氧基-鞣花酸(4)、香草醛(5)、5-羰基-四氢呋喃-3-甲酸乙酯(6)、没食子酸(7)、3,3′-二甲氧基-鞣花酸-4-(5″-乙酰基)-α-L-阿拉伯糖苷(8)、佛手柑内酯(9)。结论石油醚部位为野鸦椿根抑制肝脂堆积活性部位,化合物1、6、8和9为首次从该属植物中分离得到。
Objective To study the inhibitory effect of Euscaphis japonica root on hepatic lipid accumulation and its chemical components. Methods Different polarity fractions were prepared by extraction method. The lipid accumulation model of Hep G2 cells induced by oleic acid was observed by oil red O staining. The accumulation of intracellular lipid droplets was measured. The amount of intracellular triglyceride (TG) The active site was separated and purified from the active site by silica gel, Sephadex LH-20, HPLC and other chromatographic techniques. The structure of the compound was identified by physicochemical properties and spectral data. Results Petroleum ether fraction significantly inhibited lipid accumulation in Hep G2 cells induced by oleic acid and decreased the amount of TG. Nine compounds were isolated and identified as 3β, 19-dihydroxy-24-trans-feruloyl (1), β-sitosterol (2), 7-hydroxy-2-octene-5-lactone (3), 3,3’-dimethoxy (4), vanillin (5), ethyl 5-carbonyl-tetrahydrofuran-3-carboxylate (6), gallic acid (7), 3,3’-dimethoxy-ellagic acid- - (5) -acetyl) -α-L-arabinoside (8) and bergapten (9) .Conclusion: 9 was isolated from the genus for the first time.