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应用免疫组化ＡＢＣ法，对肝癌高发区６２例肝细胞癌组织及癌周肝组织进行了Ｐ５３和Ｃ－ｍｙｃ基因产物的标记。结果显示，肝细胞肝癌组织中Ｐ５３与Ｃ－ｍｙｃ基因蛋白均过量表达，阳性率分别为７４．１９％与６７．７４％，均高于有关文献报道的检测率。癌周肝组织中Ｐ５３与Ｃ－ｍｙｃ阳性检出率均低于癌组织。ＨＢｓＡｇ阳性病例、癌周伴肝硬变病例Ｐ５３与Ｃ－ｍｙｃ基因异常表达率均显著高于相应的阴性病例。本文随访病例中，术后肿瘤复发组Ｐ５３与Ｃ－ｍｙｃ基因表达的阳性率极明显地高于未复发尚生存组。文中还讨论了Ｐ５３和Ｃ－ｍｙｃ基因产物的表达在肝细胞癌发生中的可能作用
Immunohistochemical ABC method was used to detect the expression of P53 and C-myc gene products in 62 cases of hepatocellular carcinoma tissues and hepatic tissues in high-risk areas of hepatocellular carcinoma. The results showed that the expression of P53 and C-myc genes in hepatocellular carcinoma tissues was over-expressed. The positive rates were 74.19% and 67.74% respectively, which were higher than those reported in the literature. The positive rate of P53 and C-myc was lower in the liver tissue than in the cancer tissue. The abnormal expression rates of P53 and C-myc genes were significantly higher in HBsAg-positive cases and in peri-cancerous cirrhosis cases than in corresponding negative cases. In this follow-up case, the positive rate of P53 and C-myc gene expression in the tumor recurrence group was significantly higher than that in the non-recurrence survivor group. The possible role of P53 and C-myc gene product expression in the development of hepatocellular carcinoma is also discussed in the article.