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采用传统检测方法测定了我国北方地区美澳型核果褐腐病菌对常用杀菌剂甲基硫菌灵和新药剂啶酰菌胺的敏感性,并采用12条ISSR引物对不同抗性水平的菌株进行了分析。结果显示:74株菌株中检测出5株对甲基硫菌灵表现高抗、2株表现低抗的菌株;我国抗性菌株的突变位点与美国加州抗性菌株的突变位点相同;啶酰菌胺对来自我国和美国、新西兰、法国的45株美澳型核果褐腐病菌的EC50分布频率呈单峰曲线,EC50在0.059～0.320μg/mL之间,平均值为0.174±0.064μg/mL;基于ISSR分析得到的UPGMA聚类组与菌株的来源地及对甲基硫菌灵的抗性无相关性。
The sensitivity of the commonly used bactericides methyl thiophanate and the new drug boscalid was determined by the traditional methods in North China. Twelve ISSR primers were used to test the resistance of different strains Analysis. The results showed that among the 74 strains, 5 strains with high resistance to thiophanate-methyl and 2 strains with low resistance were detected. The mutation sites of the resistant strains in China were the same as those in the California-resistant strains in the United States. The distribution of EC50 of 45 amyloliquefaciens from America, New Zealand and France from China, USA, New Zealand and France showed single peak curve. EC50 ranged from 0.059 to 0.320 μg / mL with an average value of 0.174 ± 0.064 μg / mL. The UPGMA cluster based on ISSR analysis had no correlation with the origin of the strain and the resistance to thiophanate-methyl.