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本实验因用瘤细胞悬液法不能使实体型肿瘤转变为腹水型肿瘤,尔后采用多种不同转型方法进行转型实验,结果用诱导法转型成功地在国内首次将实体型615系小鼠可移植性肝细胞肝癌转变为腹水型肝癌株。实验结果表明,液体石蜡、RPMI1640液加1％组胺和同种全血诱导组使实体肿瘤转变为腹水型瘤株成功率高,方法简便。这为难于转成腹水型的实体肿瘤进行转型研究提供几个较实用的方法。
In this experiment, the solid tumors could not be transformed into ascites tumors because of the tumor cell suspension method. Then, a number of different transformation methods were used to carry out transformation experiments. The results were successfully transferred to the inducible method for transplantation of solid type 615 mice for the first time in China. Hepatocellular carcinoma is transformed into ascites type liver cancer. The experimental results showed that the liquid paraffin, RPMI1640 solution plus 1% histamine and the same whole blood induction group resulted in a high success rate of solid tumors transformed into ascites tumors, and the method was simple and convenient. This provides several more practical methods for transitional studies of difficult solid tumors that turn into ascites.