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人脑胶质细胞瘤是严重危害人类健康的一类恶性肿瘤.选用在DDRT-PCR研究中获得的19个代表新基因的EST之一设计筛库引物,在人cDNA文库中筛出一长度为 1535 bp的cDNA克隆,其开放读码框编码334个氨基酸,与已知蛋白无明显同源性,属一新发现的基因,GenBank接受号为AF068195.RT-PCR显示该基因产物在分化后的BT-325中的表达量明显高于分化前的细胞,暗示该蛋白可能与脑胶质细胞瘤分化有关,命名为 GDR1(Gliomadifferentiationrelatedproteinl)。
Human glioblastoma is a type of malignant tumor that seriously endangers human health. One of the 19 representative ESTs selected from the DDRT-PCR studies was designed to screen primers. A 1535 bp cDNA clone was screened in human cDNA library. The open reading frame (ORF) encoded 334 amino acids. Known protein no significant homology, is a newly discovered gene, GenBank accession number AF068195. RT-PCR showed that the expression level of this gene product in differentiated BT-325 was significantly higher than that in pre-differentiation cells, suggesting that the protein may be related to glioma differentiation, and named GDR1 (Gliomadifferentiation related protein).