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目的评价改良压碎逸蚴法接种环取样检测感染性钉螺的效果。方法按照双盲实验原则,对批量钉螺分别采用逸蚴法、压碎法和改良压碎逸蚴法检测感染性钉螺,比较3种方法漏检率和检测效率,改良压碎逸蚴法采用接种环取样,对样本表层水样中的尾蚴定性与定量。结果检测样本186份(每份100只钉螺)。改良压碎逸蚴法、逸蚴法和压碎法分别检出阳性样本32、30份和32份;逸蚴法漏检率为6.25%,压碎法和改良压碎逸蚴法无漏检;3种检测方法的检测效率分别为13.02只/min、12.95只/min和1.58只/min,改良压碎逸蚴法和逸蚴法检测效率分别是压碎法的8.24倍和8.20倍;改良压碎逸蚴法接种环取样,250 ml水样中尾蚴数≥60条时检出率为100%。结论改良压碎逸蚴法适宜疫区批量检测感染性钉螺及感染性钉螺环境(段),简便、准确、高效。
Objective To evaluate the effect of modified crushed larvae inoculation loop on the detection of infectious snails. Methods According to the principle of double-blind experiment, the infecting snails were detected by using the method of asthenospermia, crushing and modified crushing by metacercaria, respectively. The missed detection rate and the detection efficiency of the three methods were compared. Ring sampling, the sample surface water cercariae qualitative and quantitative. Results 186 samples (100 snails) were tested. Modified crushed larvae method, Jacquard larvae and crushing method were detected positive samples 32,30 copies and 32 copies; laparotomy method missed detection rate of 6.25%, Crush method and improved crushing larvae method without missing ; The detection efficiencies of the three methods were 13.02 / min, 12.95 / min and 1.58 / min, respectively. The detection rates of modified crush larvae method and metacercaria method were 8.24 times and 8.20 times respectively, Crush larvae law ring vaccination sampling, 250 ml water cercaria count ≥ 60 when the detection rate was 100%. Conclusion The improved method of crushing parasite larvae is suitable for mass detection of infecting snails and infecting snails environment (section), which is simple, accurate and efficient.