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为探讨微型土壤动物在人工林生态系统中的功能及其对环境变化的响应,利用18S rDNA宏基因组比较了湖南省攸县不同发育阶段(3、13、26年)以及不同栽培代数(1、2、3代)杉木人工林微型土壤动物的群落多样性及结构.结果表明:焦磷酸测序共得到94922条优质序列,平均长度为436 bp.杉木人工林微型土壤动物主要类群包括:脊索动物门60.8%、环节动物门24.0%、节肢动物门7.4%、线虫动物门3.6%、轮形动物门1.5%和扁形动物门1.2%.5块样地的ACE指数与Shannon指数具有显著性差异,其中第1代13年生杉木林的ACE指数(712.33)、Chao1指数(500.00)、Shannon指数(2.66)均最低,Simpson指数(0.26)最高,表明第1代13年生杉木林微型土壤动物多样性最低.相关分析发现,速效钾含量与ACE指数和Chao1指数之间存在显著负相关,而Shannon指数、Simpson指数与所测土壤理化性质之间无显著相关关系.各样地Jaccard相似性系数均<0.4,样地间微型土壤动物群落结构差异明显.
In order to investigate the function of micro-fauna in plantation ecosystem and its response to environmental changes, the 18S rDNA metagenome was used to compare the effects of different developmental stages (3, 13, 26 years) The results showed that there were 94922 high quality sequences with the average length of 436 bp.The main groups of microcagus in Chinese fir plantation include: 60.8% of the total area, 24.0% of the total fauna, 7.4% of arthropods, 3.6% of nematode animals, 1.5% of rotundity and 1.2% of flat animals.Among them, ACE and Shannon indices were significantly different The ACE index (712.33), Chao1 index (500.00), Shannon index (2.66) and the Simpson index (0.26) were the lowest in the first generation of 13 year old Chinese fir plantations, respectively, indicating that the first generation 13 - year - old Chinese fir plantations had the lowest diversity of soil micro - fauna. Correlation analysis showed that there was a significant negative correlation between available potassium content and ACE index and Chao1 index, while Shannon index and Simpson index had no significant correlation with soil physical and chemical properties. Jaccard similarity All <0.4, Community structure across samples significantly different micro-fauna.