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利用陆地棉推广品种中棉所36和海1配制杂交组合,并用中棉所36为轮回亲本构建回交群体(BC1F1,BC2F1和BC1S1)。用亲本和F1对新开发的2102对SSR引物进行多态性筛选,共筛选到317对含有海1显性带的引物,占筛选引物总数的15.08%;最终对其中的275对引物进行了BC1F1群体扩增,获得306个SSR标记差异位点。连锁分析表明(LOD=6.5),有254个标记位点连锁,分布在42个连锁群中,覆盖2252.36cM,约占棉花基因组的50.05%;平均每个连锁群有6.08个标记,覆盖53.63cM;标记间平均间距为8.87cM。利用BC1F1、BC2F1和BC1S1三个不同世代分离群体产量性状数据,共定位16个产量性状QTL,解释表型变异5.77%~19.86%。其中,衣分6个,铃重6个,籽指4个。有9个增效基因来自陆地棉亲本中棉所36,7个增效基因来自海岛棉亲本海1,说明了表型性状较差的品种同样可能含有可用于性状改良的增效基因。控制衣分的3个QTL可在不同的世代稳定检测到,效应稳定,增效基因均来自高值亲本陆地棉,为进一步分子标记辅助选择奠定了基础。
The hybrid combinations were popularized by Gossypium hirsutum 36 and Haili 1 using upland cotton, and the backcross population (BC1F1, BC2F1 and BC1S1) was constructed by using CIMC36 as the recurrent parent. Two hundred and seventy two pairs of SSR primers were screened from the parents and F1, and 317 pairs of primer pairs containing serovar 1 were screened, accounting for 15.08% of the total number of selected primers. Finally, 275 of them were subjected to BC1F1 Population amplification, access to 306 SSR marker difference sites. Linkage analysis showed that (LOD = 6.5), there are 254 marker loci linked and distributed in 42 linkage groups, covering 2252.36cM, accounting for about 50.05% of the cotton genome. On average, each linkage group has 6.08 markers covering 53.63cM The average spacing between markers was 8.87cM. The QTLs for 16 yield traits were mapped using population segregation data from BC1F1, BC2F1 and BC1S1 in three different generations to explain the phenotypic variation from 5.77% to 19.86%. Among them, the clothing points 6, 6 bell weight, seed refers to four. Nine of the synergistic genes were derived from Gossypium hirsutum (Gossypium hirsutum L.) by upland cotton (Gossypium hirsutum L.), and seven (7) synergistic genes from Gossypium barbadense sponge island (Gossypium barbadense), indicating that phenotypically poor cultivars may also contain potential synergistic genes that can be used to improve traits. The three QTLs for control of lint percentage could be detected stably in different generations and the effect was stable. The synergistic genes were all from high-yielding parent G. hirsutum, which laid the foundation for further molecular marker-assisted selection.