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目的:建立测定茅苍术中苍术苷A含量的方法,比较茅苍术生品及麸炒品中苍术苷A含量的差异。方法:采用高效液相色谱蒸发光散射检测(HPLC-ELSD)法,使用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-水(B)为流动相,梯度洗脱(0~10 min,2%A→8%A;10~15 min,8%A→15%A;15 min之后维持15%A),流速1.0 mL·min-1,柱温30℃,蒸发光散射检测器漂移管温度50℃,雾化气体为氮气,氮气压力3.0×105Pa。结果:苍术苷A进样量在0.2390~4.7800μg(r=0.9998)范围内线性关系良好;平均回收率为97.84%,RSD为1.0%。样品测定结果为0.0474%~0.0852%。结论:本方法经过方法学验证,可为茅苍术的质量控制提供实验依据。
Objective: To establish a method for determination of atractyloside A in Atractylodes macrocephalae, and to compare the content of atractyloside A in Atractylodes macrocephala and its products. METHODS: The HPLC-ELSD method was used to determine the content of arsenic in blood samples by using Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm), acetonitrile (A) -water (B) (0-10 min, 2% A → 8% A; 10-15 min, 8% A → 15% A; 15% A after 15 min) at a flow rate of 1.0 mL · min- , Evaporative light scattering detector drift tube temperature 50 ℃, atomized nitrogen gas, nitrogen pressure 3.0 × 105Pa. Results: The calibration curve of atractyloside A was good in the range of 0.2390 ~ 4.7800 μg (r = 0.9998). The average recovery was 97.84% and the RSD was 1.0%. The result of the sample measurement is 0.0474% ~ 0.0852%. Conclusion: This method is validated by methodology, which can provide experimental evidence for the quality control of Atractylodes.