【摘 要】
：
Influenza epidemics frequently and unpredictably break out all over the world, and seriously affect the breeding industry and human activity. In-activated and l
【机 构】
：
Department of Life Science,Pohang University of Science and Technology,Pohang 37673,Korea;College of
论文部分内容阅读
Influenza epidemics frequently and unpredictably break out all over the world, and seriously affect the breeding industry and human activity. In-activated and live attenuated viruses have been used as protective vaccines but exhibit high risks for biosafety. Subunit vaccines enjoy high bio-safety and specificity but have a few weak points compared to inactivated virus or live attenuated virus vaccines, especially in low immunogenicity. In this study, we developed a new subunit vaccine platform for a potent, adjuvant-free, and multivalent vaccination. The ectodomains of he-magglutinins (HAs) of influenza viruses were ex-pressed in plants as trimers (tHAs) to mimic their native forms. tHAs in plant extracts were directly used without purification for binding to inactivated Lactococcus (iLact) to produce iLact-tHAs, an antigen-carrying bacteria-like particle (BLP). tHAs BLP showed strong immune responses in mice and chickens without adjuvants. Moreover, si-multaneous injection of two different antigens by two different formulas, tHAH5N6 + H9N2 BLP or a combination of tHAH5N6 BLP and tHAH9N2 BLP, led to strong immune responses to both antigens. Based on these results, we propose combinations of plant-based antigen production and BLP-based delivery as a highly potent and cost-effective platform for multivalent vaccination for subunit vaccines.
其他文献
Agricultural science and technology parks(ASTPs)represent an important growth pole in China's agricultural modernization.Clarifying their diffusion laws can op
DNA cytosine methylation confers stable epigenetic silencing in plants and many animals.However,the mechanisms underlying DNA methylation-mediated genomic silencing are not fully understood.We conducted a forward genetic screen for cellular factors requir
Pure red cell aplasia(PRCA) is a well-recognized complication of ABO major mismatched allogeneic hematopoietic stem cell transplantation(allo-HSCT), with a reported incidence of 10% – 20%(Zhidong et al., 2012;Busca et al., 2018). It is clinically characte
Hemin can improve the stress resistance of plants through the heme oxygenase system.Additionally,substances contained in plants,such as secondary metabolites,can improve stress resistance.However,few studies have explored the effects of hemin on secondary
MicroRNAs(miRNAs)play key roles in the post-transcriptional regulation of gene expression in plants.Many miRNAs are responsive to environmental signals.Light is the first environmental signal perceived by plants after emergence from the soil.However,less
Lonicera japonica Thunb.,a traditional Chinese herb,has been used for treating human diseases for thousands of years.Recently,the genome of L.japonica has been decoded,providing valuable information for research into gene function.However,no comprehensive
矩阵求逆作为线性代数的一个重要研究内容,在通信工程、计算机、物理学等领域有着广泛的应用。本文探讨了针对矩阵求逆的一类位移分裂迭代算法,同时分析了该方法在求逆矩阵时的收敛性,并用具体的数值实验验证了该方法的可行性。
对于增广拉格朗日乘子法,分析表明其解析解只有一个不等式约束的边界解严格成立,而其在可行域内的解析解在松弛变量为实数时存在,当松弛变量为虚数时不等式约束不满足,解析解不在可行域内,增广拉格朗日乘子法无效。当采用无约束最优化算法求解数值解时,在一定的条件下数值解在可行域内,增广拉格朗日乘子法有效,若条件不成立,则增广拉格朗日乘子法无效。本文在增广拉格朗日函数中加入松弛因子,修正了增广拉格朗日乘子法,使其具有一个在可行域内的严格成立的解析解,当松弛因子与终止条件的允许误差限满足一定的条件时,不等式约束满足,修正
本文通过对原研品进行逆向工程分析,优化处方,制备出质量和疗效与原研品一致的吸入用布地奈德混悬液。方法:采用显微镜法测定原料药和原研品的粒径,根据原研品公布信息确定自制品所用辅料,以振摇次数为考核指标,采用正交实验法确定最佳制备工艺,并用中试实验对所选工艺进行验证。结果表明,原料药与原研品粒径相似,选用与原研品一致的辅料进行自制品的制备研究,确定的最佳处方在中试条件下重复性良好,自制品质量符合标准要求,稳定性实验结果良好。
Social assistance is the last safety net in the social security system and plays a vital role in poverty alleviation in countries around the world. Promoting the equal financial assistance is meaningf