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目的探讨阿托伐他汀对IL-6诱导的人脐静脉内皮细胞表达内皮抑素(ES)的抑制作用。方法采用IL-6和/或阿托伐他汀处理人脐静脉内皮细胞24～48h,酶联免疫吸附测定法(ELISA)检测培养人脐静脉内皮细胞上清液中ES浓度,RT-PCR检测ESmRNA的表达。结果 IL-6组48h时较对照组上清液ES浓度显著增加[(18.45±6.80)vs(6.81±2.33)ng/ml,P<0.01],同时较24h时上清液ES浓度显著增加[(18.45±6.80)vs(7.07±2.54)ng/ml,P<0.01];At+IL-6组与IL-6组48h时比较上清液ES浓度减少差异有统计学意义[(9.82±4.74)vs(18.45±6.80)ng/ml,P<0.05]。IL-6组48h时与对照组比较ESmRNA表达显著增加[(0.587±0.220)vs(0.122±0.096),P<0.001];At+IL-6组48h时与IL-6组相比ESmRNA表达减少差异有统计学意义[(0.310±0.205)vs(0.587±0.220),P<0.05]。结论 IL-6可诱导人脐静脉内皮细胞分泌表达ES,阿托伐他汀对其分泌表达ES具有抑制作用。
Objective To investigate the inhibitory effect of atorvastatin on the expression of endostatin (ES) induced by IL-6 in human umbilical vein endothelial cells. Methods Human umbilical vein endothelial cells (ILs) were treated with IL-6 and / or atorvastatin for 24-48 h. The concentrations of ES in culture supernatants of human umbilical vein endothelial cells were detected by enzyme-linked immunosorbent assay (ELISA) expression. Results Compared with control group, the concentration of ES in supernatant of IL-6 group increased significantly at 48h ([(18.45 ± 6.80) vs (6.81 ± 2.33) ng / ml, P <0.01] (18.45 ± 6.80) vs (7.07 ± 2.54) ng / ml, P <0.01]. There was a significant difference in the concentration of ES in supernatant between At + IL-6 group and IL-6 group at 48h [(9.82 ± 4.74 ) vs (18.45 ± 6.80) ng / ml, P <0.05]. Compared with the control group, the expression of ES mRNA in IL-6 group was significantly increased at 48h compared with the control group [(0.587 ± 0.220) vs (0.122 ± 0.096, P <0.001] The difference was statistically significant [(0.310 ± 0.205) vs (0.587 ± 0.220), P <0.05]. Conclusion IL-6 can induce the secretion of ES in human umbilical vein endothelial cells, and atorvastatin has an inhibitory effect on the secretion of ES.