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目的研究盾叶薯蓣水难溶性提取物的化学成分及对人肝癌细胞HepG2生长的抑制作用。方法采用正、反相硅胶柱色谱方法分离纯化水难溶性提取物的单体化合物,并通过波谱学方法鉴定其结构;采用MTT法评价其体外抑制人肝癌细胞的增殖作用。结果采用化学方法分离并鉴定了5个化合物,分别是薯蓣皂苷元(Ⅰ)、延令草皂苷(Ⅱ)、薯蓣皂苷元纤维二糖苷(Ⅲ)、三角叶皂苷(Ⅳ)和盾叶皂苷I(Ⅴ);5个化合物对人肝癌细胞的增值具有明显的抑制作用,其中化合物Ⅴ对人肝癌细胞HepG2的生长抑制作用最强。结论盾叶皂苷Ⅰ具有明显的抗癌活性。
Objective To study the chemical constituents and the inhibitory effect on the growth of HepG2 human hepatocellular carcinoma cells. Methods The compounds were isolated and purified by normal and reverse phase silica gel column chromatography. Their structures were identified by spectroscopic methods. The proliferation of human hepatoma cells was evaluated by MTT assay. Results Five chemical compounds were isolated and identified as diosgenin (Ⅰ), saponins (Ⅱ), diosgenin (Ⅲ), saponin (Ⅳ) and saponin I (Ⅴ). Five compounds have obvious inhibitory effect on the proliferation of human hepatocellular carcinoma cells, and compound Ⅴ has the strongest inhibitory effect on HepG2 human hepatoma cells. Conclusion Aegilini I has obvious antitumor activity.