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目的 探讨多聚赖氨酸修饰的氧化铁纳米粒子(Fe_2O_3-PLL)标记的小鼠P388D1细胞经静脉注射后对中枢Wallerian变性的趋向性.材料与方法制备中枢神经顺行溃变大鼠模型,用Fe_2O_3-PLL标记的P388D1细胞通过大鼠尾静脉注射人体内,分别于皮层毁损后8 h和2天和4天进行MR T_2WI,活体跟踪Fe_2O_3-PLL标记的P388D1细胞对中枢神经系统Wallerian变性的趋向性.结果 皮层毁损后8 h在T_2WI上无观察到毁损局部及其他区域的异常信号;2天后可在T_2WI观察到毁损局部脑区及沿胼胝体分布的点状低信号,脊髓未见异常信号;皮层毁损后4天可见颈髓后索和延髓锥体束的点状低信号.结论 运用Fe_2O_3-PLL标记的外源性巨噬细胞可以观察到中枢Wallerian变性的发作进展,并为运用外源性巨噬细胞作为重组基凶的运载细胞进行基因治疗提供了研究基础.“,”Objective To investigate murine monocytes/macrophages(Mo/Ma) labeled with micrometer-sized particles of Fe_2O_3 PLL targeted Wallerian degeneration of central nerve system(CNS).Materials and Methods 8 male Sprague Dawley rats were used to establish wallerian degeneration model of CNS by infracortical axotomy.The labeled Mo/Ma administered intravenously to target Wallerian degeneration at different time.Results 8h after infracortical axotomy,high signals were observed in injury area on T_2WI.2 days later,low signals were observed in injury area and corpus callosum.4 days later low signals were observed in posterior funiculus of cervical cord and fasciculi pyramidales medullae oblong-atae.Conclusion The labeled Mo/Ma can be used to trace wallerian degeneration of CNS non-invasively with T_2-weighted MRI The results suggest that Mo/Ma may be applyed for genic therapy.