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目的:探讨Ki-67、p16INK4a和人乳头状瘤病毒(HPV)在不同程度宫颈鳞状上皮病变组织中的表达及临床病理意义。方法:采用原位分子杂交及免疫组化方法,检测HPV的不同亚型、Ki-67、p16INK4a蛋白在182例不同程度宫颈病变组织中的表达。结果:HPV在不同程度病变中总检出率52.19%(95/182);在宫颈高级别上皮内瘤变及鳞癌组中检出最多的感染类型为HPV16/18,而HPV6/11在尖锐湿疣组检出率87.50%(21/24)最高;随着宫颈病变严重程度的增加,级别升高,Ki-67、p16INK4a阳性程度呈递增趋势。Ki-67、p16INK4a与HPV16/18型感染关系密切,χ2=11.779 8,P<0.01;Ki-67也与HPV6/11型有关。结论:HPV16/18型及Ki-67、p16INK4a在宫颈高级别上皮内瘤变及鳞癌中表达明显升高,可能对宫颈鳞癌的发生、发展具有协同作用。
Objective: To investigate the expression and clinicopathological significance of Ki-67, p16INK4a and human papillomavirus (HPV) in cervical squamous cell carcinoma of different degree. Methods: In situ hybridization and immunohistochemistry were used to detect the expression of different subtypes of HPV, Ki-67 and p16INK4a in 182 cases of cervical lesions. Results: The overall detection rate of HPV was 52.19% (95/182) in different degree of lesions. HPV16 / 18 was the most frequently detected type in high grade cervical intraepithelial neoplasia and squamous cell carcinoma, while HPV6 / 11 was found to be sharp The detection rate of condyloma acuminatum was the highest (87.50% (21/24)). As the severity of cervical lesions increased, the grade of Ki-67 and p16INK4a tended to increase. Ki-67, p16INK4a and HPV16 / 18 infection were closely related, χ2 = 11.779 8, P <0.01; Ki-67 also associated with HPV6 / 11 type. Conclusion: The expression of HPV16 / 18 and Ki-67, p16INK4a in cervical intraepithelial grade high grade intraepithelial neoplasia and squamous cell carcinoma are obviously increased, which may have a synergistic effect on the occurrence and development of cervical squamous cell carcinoma.