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应用傅里叶变换红外(Fourier transform infrared,FTIR)光谱技术研究性别、年龄、组织离体等因素对死亡时间(PMI)的影响。将SD 大鼠分别设置雌雄组、同性别不同年龄组(21 d 组、42 d 组、63 d 组)、同性别在体与离体组。采用颈椎脱臼法处死大鼠,保存在稳定条件[温度(20±2)℃,湿度50%]下。选择死后即刻至48 h 不同时间点获取大鼠肝、肾、脾、心肌、脑、肺、骨骼肌等组织,采用FTIR 光谱技术进行检测和分析。随着PMI 推移,各组大鼠组织的FTIR 光谱主要吸收峰峰位及其比值没有因为性别、年龄、组织离体等而出现显著性差异。性别、年龄、组织离体等不是影响FTIR 光谱技术推断PMI 的关键因素,在今后的动物与人体实验中可忽略其对PMI 的影响。
Fourier transform infrared (FTIR) spectroscopy was used to study the influence of gender, age, tissue isolation and other factors on death time (PMI). The SD rats were set male and female groups, respectively, of the same sex in different age groups (21d group, 42d group, 63d group), same sex in vivo and in vitro groups. Rats were sacrificed by cervical dislocation and stored under stable conditions [temperature (20 ± 2) ° C, humidity 50%]. The liver, kidney, spleen, myocardium, brain, lung, skeletal muscle and other tissues were obtained at different time points immediately after the death to 48 h. FTIR spectroscopy was used for detection and analysis. With the passage of PMI, there was no significant difference in the main absorption peaks and peak ratios of the FTIR spectra of rats in each group due to gender, age, tissue isolation and so on. Sex, age, tissue isolation, etc. are not the key factors that influence the PMI estimation by FTIR spectroscopy. Their effects on PMI may be neglected in animal and human experiments in the future.