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目的:建立HPLC同时测定皖贝止咳滴丸中贝母素甲、贝母素乙及异贝母素甲含量的方法。方法:采用CAPCELL PAK C_(18)色谱柱,柱温35℃,流动相乙腈-0.1%三乙胺溶液(70∶30),流速1.0 m L·min~(-1);ELSD参数为漂移管温度95℃,载气流速2.2 m L·min~(-1)。结果:贝母素甲、贝母素乙及异贝母素甲分别在34.33~343.3,30.17~301.7,82.11~821.1 mg·L~(-1)线性关系良好;贝母素甲的平均加样回收率为97.55%,RSD 1.3%,贝母素乙的平均加样回收率为97.99%,RSD 1.8%,异贝母素甲的平均加样回收率为98.42%,RSD 1.4%。结论:该法简便、准确、可靠,可作为皖贝止咳滴丸质量控制的方法。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of peimine, peiminine and paeoniflorin in Wanbei Zhike Drop Pills. Methods: CAPCELL PAK C_ (18) column was used. The column temperature was 35 ℃ and the mobile phase was acetonitrile-0.1% triethylamine solution (70:30). The flow rate was 1.0 m L · min -1. The temperature was 95 ℃ and the carrier gas flow rate was 2.2 m L · min ~ (-1). Results: There was a good linear relationship between Fritillariae, Fritillaria B and Fritillaria in 34.33 ~ 343.3,30.17 ~ 301.7,82.11 ~ 821.1 mg · L -1, The average recoveries were 97.55% and RSD 1.3%. The average recoveries of peptone B were 97.99% and RSD 1.8%, respectively. The average recoveries of paeoniflorin A were 98.42% and 1.4% respectively. Conclusion: The method is simple, accurate and reliable and can be used as a quality control method for Wanbei Zhike Dropping Pills.