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采用 γ－Ａｌ_２Ｏ_３因相萃取和 ＨＰＬＣ法对云南红豆衫和东北红豆杉培养细胞中的紫杉醇进行了定量分析。红豆杉浸膏粗品经γ－Ａｌ_２Ｏ_３因相萃取柱后，可直接上高效液相色谱柱。Ｃ_(１８) ＨＰＬＣ的最佳条件是：甲醇－乙腈－水（２５：４５：３０）为流动相，２２７ ｍｍ紫外检测。该法简单、快速、准确、适应性强，对紫杉醇的检测范围在０．４～１９．２ μｇ。
Quantitative analysis of paclitaxel in cultured cells of Taxus cuspidata and Taxus cuspidata with γ-Al 2 O 3 phase extraction and HPLC was carried out. Crude extract of Taxus by γ-Al_2O_3 phase extraction column can be directly on the high performance liquid chromatography column. The optimal conditions of C_ (18) HPLC were methanol - acetonitrile - water (25:45:30) as mobile phase and 227 mm UV detection. The method is simple, rapid, accurate and adaptable. The detection range of paclitaxel is 0.4 ~ 19.2 μg.