目的探讨青蒿琥酯(Art)对双倍体卫氏并殖吸虫病的治疗作用。方法1)卫氏并殖吸虫成虫用含不同浓度Art的RPMI 1640培养基体外培养后作电镜观察;2)实验犬常规感染卫氏并殖吸虫囊蚴,76 d后用青蒿琥酯50和80mg/kg灌胃治疗,103 d解剖犬,作常规病理检查,取出的虫体作电镜观察。结果1)5 mg/kg组体外培养24 h后,虫体萎缩,扫描电镜观察部分体棘消失、倒伏、絮状物附着;透射电镜显示皮层水肿、细胞器及分泌颗粒减少,卵黄腺萎缩,卵巢卵细胞胞浆空泡变等;2)两治疗犬体内虫体萎缩,厚壁囊肿数分别为28和35个,对照犬为16个,差异有显著性(P<0.05);透射电镜显示成虫变化与体外培养试验相似。结论青蒿琥酯对卫氏并殖吸虫有损伤作用。 Objective To investigate the therapeutic effect of artesunate on the diploid Paragonimus westermani disease. Methods 1) Paragonimus westermani adults were cultured in vitro with RPMI 1640 medium containing different concentrations of Art; 2) Experimental dogs were routinely infected with Paragonimus westermani metacercaria, and after 76 days, artesunate 50 and 80mg / kg gavage treatment, 103d dissected dogs for routine pathological examination, remove the parasites for electron microscopy. The results showed that: 1) The worms were atrophied after 5 mg / kg in vitro for 24 h, and some spines disappeared, lodging and flocs were observed by scanning electron microscopy. Transmission electron microscopy showed cortical edema, decreased organelles and secretory granules, atrophy of the yolk glands, (2) There were 28 and 35 thick wall cysts in the two treated dogs and 16 in control dogs, respectively (P <0.05). Transmission electron microscopy showed changes in adults Similar to in vitro culture experiments. Conclusion Artesunate has an injury effect on Paragonimus westermani.