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目的建立SD大鼠正己烷(-—hexane)吸入染毒实验模型,为研究毒物对肺、肝和肾等重要器官的损害提供方法学依据。方法24只雄性SD大鼠随机分为4组,每组6只,分为正常对照组和3个染毒组(分别吸入染毒1、3和7 d),置于特制染毒箱中,正己烷初始浓度设为35．2 g／m3,静式染毒。各染毒组大鼠均于染毒结束后30 min内取动脉血,由气相色谱-质谱仪检测全血正己烷浓度,同时新鲜组织取材后常规石蜡包埋,切片经HE染色,作病理学检查。结果染毒1、3和7 d组大鼠血液正己烷平均浓度分别为:636．92、913．49和1090．69μg／L,3组浓度均明显高于对照组,表明已达到染毒要求。显微镜观察发现,实验大鼠肺、肝和肾等主要器官的微细结构均出现明显的病理学变化。结论SD大鼠以正己烷35．2 g／m3为初始浓度的静式吸入染毒方法,可以成功建立正己烷吸入致肺、肝和肾损伤的实验模型,操作简便并具重复性,具有一定的科学性和可行性。
OBJECTIVE: To establish an experimental model of inhalation of n-Hexane in SD rats to provide a methodological basis for studying the damage of toxicants to vital organs such as lung, liver and kidney. Methods Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups with 6 rats in each group. The rats were divided into normal control group and 3 exposure groups (1,3 and 7 d inhalation respectively) The initial concentration of n-hexane was 35.2 g / m3, which was then exposed to the static. Blood samples were taken from the rats in each exposure group within 30 min after the end of the treatment. The concentrations of n-hexane in whole blood were determined by gas chromatography-mass spectrometry (GC-MS). The fresh tissues were embedded in paraffin and paraffin sections were stained with HE for histopathology an examination. Results The mean concentrations of n-hexane in blood were 636.92, 913.49 and 1090.69μg / L respectively in the 1,3 and 7 d groups, and the concentrations in the three groups were significantly higher than those in the control group . Microscopic observation showed that the pathological changes of the microstructure of major organs such as lung, liver and kidney were observed in experimental rats. Conclusion The experimental inhalation of n-hexane into lung, liver and kidney can be successfully established in SD rats by inhalation of n-hexane at a concentration of 35.2 g / m3 as initial concentration. The method is simple, reproducible and reproducible. Scientific and feasible.