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为了建立适宜的胚胎体外培养体系，本试验对小鼠２－细胞胚胎体外发育及其影响因素进行了研究。结果表明，２－细胞胚胎在供试的三种培养基中均可进一步发育；血清、ＣＯ２气体纯度，培养基水的纯度等均可影响到胚胎的发育，从而优化了胚胎体外培养的实验室条件和方法。
In order to establish a suitable embryo culture system in vitro, we studied the in vitro development of mouse 2-cell embryos and its influencing factors. The results showed that 2-cell embryos could be further developed in all three media tested. The purity of serum gas, CO2 gas and water of culture medium all influenced the development of embryos and optimized the embryo culture laboratory Conditions and methods.