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在携带ＣａＭＶＣａｂｂＢ－ＪＩ株系基因Ⅵ的根癌农杆菌菌种ＧＶ３１０１的介导下，对大白菜、油菜幼苗生理状态、真空渗入时间及不同处理部位和叶面造伤等的处理与遗传转化效果的关系进行了比较研究。结果表明，经自然春化后，大白菜、白菜型油菜植株长至４～５片叶，甘蓝型油菜、芥菜型油菜植株长至７～９片叶，花薹已抽出时，为真空渗入最适苗态。大白菜自然春化植株移栽缓苗（３ｄ），或者黑暗处理（８ｈ）均可显著提高其转化率。在吸力为１４．４ｍ３／ｈ的真空泵下处理１ｍｉｎ后，迅速打开真空泵气阀升压变常压，再关闭阀门抽真空３０ｓ的转化效果较好。仅处理叶片转化率可高达１１．２０％，叶面造伤处理对转化率没有明显影响。
Under the guidance of Agrobacterium tumefaciens strain GV3101 carrying gene Ⅵ of CaMVCabbB-JI strain, the effects of treatment and genetic transformation on the physiological status, the time of vacuum infiltration, the different treatment sites and foliar injury of Chinese cabbage and rape The relationship between the comparative study. The results showed that after natural vernalization, the plants of Chinese cabbage and Chinese cabbage were 4 to 5 leaves in length. The plants of Brassica napus and Brassica juncea were 7 to 9 leaves in length. Suitable for Miao state. The natural vernalization of cabbage transplanting Huanmiao (3d), or dark treatment (8h) can significantly improve the conversion rate. In the suction of 14.4m3 / h vacuum pump for 1min, quickly open the vacuum pump valve pressure booster pressure, and then close the valve vacuum 30s better conversion. Only leaf conversion rate can be as high as 11.20%, leaf injury treatment has no significant effect on the conversion rate.