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研究酪氨酸激酶相关蛋白2(tyrosinase related protein 2,TRP-2)和gp100质粒DNA在B16黑色素瘤小鼠模型中的抗肿瘤作用。TRP-2及gp100在人类和鼠类黑色素瘤中均高度表达。鼠源gp100(mgp100)和鼠源TRP-2(mTRP-2)在小鼠中的免疫原性较差,而异源黑色素瘤相关抗原可打破这些免疫耐受。使用电脉冲法免疫人源gp100(hgp100)和人源TRP-2(hTRP-2)质粒在B16F10肿瘤模型中表现出显著的保护作用。TRP-2和gp100质粒免疫结合Ii-PADRE(invariant Pan DR reactive epitope)和鼠源白介素-12(murine interleukin-12,mIL-12)质粒有效消退了建立的皮下B16F10肿瘤。上述结果表明,肌肉内注射异源DNA疫苗结合Ii-PADRE与IL-12质粒使用可能是一种有效治疗黑色素瘤的策略。
To study the antitumor effect of tyrosine kinase related protein 2 (TRP-2) and gp100 plasmid DNA in B16 melanoma mouse model. TRP-2 and gp100 are highly expressed in both human and murine melanomas. The immunogenicity of murine gp100 (mgp100) and murine TRP-2 (mTRP-2) is poor in mice, whereas allogeneic melanoma-associated antigens can break these immune tolerance. Immunization of human gp100 (hgp100) and human TRP-2 (hTRP-2) plasmids using the electrical pulse method showed a significant protective effect in the B16F10 tumor model. The TRP-2 and gp100 plasmids immunostained for the established subcutaneous B16F10 tumors immunostaining for the invariant Pan DR reactive epitope and murine interleukin-12 (mIL-12) plasmids. These results suggest that the intramuscular injection of a heterologous DNA vaccine in combination with the use of Ii-PADRE and IL-12 plasmids may be a strategy for the effective treatment of melanoma.