以TC-100、Grace's作为昆虫细胞低血清或无血清培养的基础培养基时,需补加一定量的取代因子脂类复合物、激素以及微量元素等。为了取代高成本的进口商业化酯类复合物,将从鸡蛋黄中提取的磷脂配制成蛋黄脂乳液,用于棉铃虫细胞HzAm1的驯化及浓度测定。结果表明:最大细胞浓度提高了31.7%,比单纯TC—100培养基提高了117.2%,其改进效果明显。 To TC-100, Grace's as insect cell serum or serum-free culture of the basic medium, the need to add a certain amount of substitution factor lipid complexes, hormones and trace elements. In order to replace the high-cost imported commercial ester complex, the phospholipids extracted from egg yolk were formulated into egg white lipid emulsion for domestication and concentration determination of the cotton bollworm cell HzAm1. The results showed that the maximum cell concentration increased by 31.7%, which was 117.2% higher than that of TC-100 medium alone. The improvement effect was obvious.