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目的:探讨柴胡皂苷d(SSd)对二甲基亚硝胺(DMN)致肝纤维化大鼠血清白细胞介素-10(IL-10)、肿瘤坏死因子(TNF-α)、一氧化氮(NO)水平的影响。方法:将SD大鼠随机分为正常组、模型组和SSd治疗组。模型组和SSd治疗组以DMN腹腔内注射建立肝纤维化模型,每周2次,持续6周。SSd治疗组注射DMN2周后同时每天腹腔注射SSd治疗4周。正常组每天腹腔注射生理盐水,持续6周。观察大鼠血清中IL-10、TNF-α、NO水平,检测血清转氨酶含量,并对肝组织进行病理检查。结果:经SSd治疗后,大鼠肝纤维化的程度明显减轻,体内ALT和AST水平显著下降(P<0.05),并能够升高肝纤维化大鼠血清中IL-10、NO水平(P<0.05),降低血清中过高的TNF-α水平(P<0.01)。结论:SSd有明显的保护肝细胞,抗肝纤维化的作用,其机制可能与IL-10、TNF-α、NO有关。
Objective: To investigate the effects of saikosaponin d (SSd) on serum interleukin-10 (IL-10), tumor necrosis factor (TNF-α), nitric oxide (NO) levels. Methods: SD rats were randomly divided into normal group, model group and SSd treatment group. The model group and the SSd treatment group were injected with DMN intraperitoneally to establish the hepatic fibrosis model twice a week for 6 weeks. SSd treatment group were injected with DMN for 2 weeks and intraperitoneal injection of SSd for 4 weeks. The normal group was injected intraperitoneally with normal saline for 6 weeks. The levels of IL-10, TNF-α and NO in serum of the rats were observed, the content of serum aminotransferase was detected, and the pathological examination of the liver tissue was performed. Results: After treatment with SSd, the degree of hepatic fibrosis was significantly reduced, the levels of ALT and AST were significantly decreased (P <0.05), and the levels of IL-10 and NO in serum of rats with hepatic fibrosis were increased (P < 0.05), lower the level of serum TNF-α (P <0.01). Conclusion: SSd has a significant protective effect on hepatocytes against hepatic fibrosis, and its mechanism may be related to IL-10, TNF-α and NO.