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目的:探讨不同分化的胃癌细胞对不同化疗药物的敏感性。方法:选择低分化的MGC-803和高分化的AGS两种胃癌细胞系,以及正常胃黏膜上皮细胞GES-1,分别加入不同浓度的5-氟尿嘧啶(5-FU)、奥沙利铂(L-OHP)、多西他赛(DXT)、顺铂(DDP)、伊立替康(CPT-11)作用48 h后,用MTT法检测药物对细胞的抑制作用,并计算药物半数抑制浓度(IC50)。用IC50的5-FU、DDP、L-OHP作用于MGC-803和AGS细胞48 h后,检测细胞凋亡及细胞周期分布情况。结果:5种化疗药物对MGC-803、AGS细胞以及GES-1细胞均有明显的抑制作用(均P<0.05),其中5-FU、DDP、CPT-11对AGS细胞的作用较强;L-OHP、DXT对MGC-803细胞作用较强;DDP对GES-1细胞的抑制作用较强。5-FU、DDP、L-OHP均明显诱导两种胃癌细胞凋亡,其中5-FU组与L-OHP组凋亡率高于DDP组(均P<0.05);细胞周期分析显示,L-OHP增加两种细胞的S期阻滞,DDP增加MGC-803细胞的S期阻滞,5-FU与DDP增加AGS细胞的G1阻滞(均P<0.05),但5-FU对MGC-803细胞周期无明显影响(P>0.05)。结论:化疗药物对胃癌细胞的抑制作用与其病理类型有关,且对胃癌细胞凋亡的诱导和细胞周期阻滞作用均不同。
Objective: To investigate the sensitivity of different differentiated gastric cancer cells to different chemotherapy drugs. METHODS: Two gastric cancer cell lines with poorly differentiated MGC-803 and well-differentiated AGS cells and normal gastric epithelial cells GES-1 were selected and treated with 5-fluorouracil (5-FU), oxaliplatin -OHP, DXT, CPT-11 for 48 h, the inhibitory effect of the drug on the cells was measured by MTT assay and the half-maximal inhibitory concentration (IC50 ). MGC-803 and AGS cells were treated with IC50 of 5-FU, DDP and L-OHP for 48 h, and the apoptosis and cell cycle distribution were detected. Results: The five chemotherapeutic drugs significantly inhibited MGC-803, AGS cells and GES-1 cells (all P <0.05), of which 5-FU, DDP and CPT-11 had stronger effects on AGS cells. -OHP, DXT had stronger effect on MGC-803 cells; DDP had stronger inhibitory effect on GES-1 cells. 5-FU, DDP and L-OHP significantly induced apoptosis in both gastric cancer cell lines. The apoptosis rates in 5-FU and L-OHP groups were higher than those in DDP group (all P <0.05). Cell cycle analysis showed that L- OHP increased S phase arrest of both cells, DDP increased S phase arrest of MGC-803 cells, G1 arrest of AGS cells was enhanced by 5-FU and DDP (all P <0.05) No significant effect on cell cycle (P> 0.05). CONCLUSION: The suppressive effect of chemotherapeutic drugs on gastric cancer cells is related to their pathological types, and their effects on the induction of apoptosis and cell cycle arrest of gastric cancer cells are different.