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目的探讨根皮苷对血吸虫病肝纤维化小鼠肝组织中转化生长因子β1(TGF-β1)和人血小板衍生生长因子BB(PDGF-BB)表达的影响。方法用腹部贴片法感染昆明小鼠,建立血吸虫病肝纤维化模型,第6周末,肝纤维化模型建立;用吡喹酮杀虫两天后,用低、中、高剂量根皮苷(10、20、40 mg·kg-1·d-1)治疗,同时设病理模型组、空白对照和阳性对照(INF-γ)组;给药8周后,采用免疫组织化学检测各组大鼠肝脏组织中TGF-β1和PDGF-BB的表达,做HE染色测量血吸虫的虫卵结节。结果 TGF-β1和PDGF-BB含量在用药第4周到第8周呈降低趋势,且高剂量根皮苷组的各项指标最接近空白组,抑制血吸虫病肝纤维化作用明显;HE染色显示高剂量根皮苷组的肝脏变性坏死减轻明显,小叶结构完整;高剂量根皮苷组的TGF-β1和PDGF-BB含量与模型组、吡喹酮组、低剂量根皮苷组和中剂量根皮苷组的都有显著性差异(P<0.01);与IFN-γ组比较无显著性差异(P>0.05);根皮苷各剂量组和IFN-γ组的计分较模型组显著降低(P<0.01),根皮苷低、中量组的计分与IFN-γ组的比较有显著性差异(P<0.05),根皮苷各剂量组之间比较有显著性差异(P<0.05)。结果表明:根皮苷剂量呈量效增强关系。结论根皮苷能降低血吸虫病肝纤维化小鼠肝脏中TGF-β1和PDGF-BB的表达,使血吸虫虫卵结节减小,使血吸虫病肝纤维化程度减轻。
Objective To investigate the effects of phlorizin on the expression of transforming growth factor β1 (TGF-β1) and human platelet-derived growth factor BB (PDGF-BB) in the liver of schistosomiasis mice with liver fibrosis. Methods Kunming mice were infected by abdominal patch method to establish a model of hepatic fibrosis induced by schistosomiasis. At the end of the 6th week, the model of hepatic fibrosis was established. After two days of treatment with praziquantel, the low, medium and high doses of phlorizin (10 , 20,40 mg · kg-1 · d-1). The pathological model group, blank control group and positive control group (INF-γ) were also established. After 8 weeks of administration, the liver of each group was detected by immunohistochemistry The expression of TGF-β1 and PDGF-BB in the tissue was measured by HE staining for the egg nodules of schistosomiasis. Results The contents of TGF-β1 and PDGF-BB decreased from the 4th week to the 8th week, and the indexes of high-dose phlorizin group were the closest to the blank group, and the inhibitory effect on hepatic fibrosis of schistosomiasis was obvious. HE staining showed high In the high-dose phlorizin group, the levels of TGF-β1 and PDGF-BB in the phlorizin group were significantly reduced compared with the model group, the praziquantel group, the low-dose phlorizin group, and the middle-dose root (P <0.01). Compared with IFN-γ group, there was no significant difference (P> 0.05). The scores of phlorizin in each dose group and IFN-γ group were significantly lower than those in model group (P <0.01). The scores of phlorizin in low and middle dose group were significantly different from those in IFN-γ group (P <0.05), and there was significant difference between phlebitin dose groups (P < 0.05). The results showed that the dose of phlorizin increased in a dose-effect manner. Conclusion Phloridzin can reduce the expression of TGF-β1 and PDGF-BB in the liver of schistosomiasis mice with liver fibrosis and decrease the egg nodules of Schistosoma japonicum and reduce the degree of liver fibrosis in schistosomiasis.