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目的:探讨重编程相关因子Oct-4、Sox2和c-Myc在大鼠牙髓组织和牙周韧带组织损伤修复过程中的表达及作用。方法:建立大鼠牙髓牙周联合损伤动物模型,HE染色观察4周后牙髓牙本质复合体及牙周附着装置修复情况,免疫组织荧光染色检测重编程相关因子Oct-4、Sox2和c-Myc在牙髓和牙周联合损伤修复动物体内模型的表达和分布。结果:HE染色显示,大鼠牙髓牙周联合损伤4周后,新生的牙髓牙本质复合体及牙周附着装置形成;免疫荧光显示,在体内牙髓牙周组织损伤修复的过程中,Oct-4、Sox2和c-Myc在牙髓及牙周损伤修复的新生组织均明显激活,Oct-4和Sox2呈现相似的表达趋势。结论:Oct-4、Sox2和c-Myc均参与体内牙髓牙本质复合体和牙周附着装置的损伤修复,Oct-4和Sox2呈现相似的表达趋势,提示Sox2和Oct-4可能存在协同调控作用,而c-Myc可能具备独立的功能。
OBJECTIVE: To investigate the expression and the role of reprogramming related factors Oct-4, Sox2 and c-Myc in rat pulp tissue and periodontal ligament tissue during the repair process. Methods: The animal model of rat periodontal ligament damage was established. HE staining was used to observe the repair of dental pulp dentin complex and periodontal attachment device. Immunofluorescence staining was used to detect the reprogramming related factors Oct-4, Sox2 and c-Myc Expression and distribution of the model in vivo in pulp and periodontal injury-repaired animals. Results: The HE staining showed that the newly formed odontoblastic dentin complex and periodontal attachment were formed after the periodontal ligament injury in rats. Immunofluorescence showed that in the process of periodontal tissue injury repair in vivo, Oct-4, Sox2 and c-Myc were significantly activated in pulp and periodontal injury-repaired neoplasms, and Oct-4 and Sox2 showed similar expression trends. Conclusions: Both Oct-4, Sox2 and c-Myc are involved in the injury and repair of dental pulp dentin complex and periodontal attachment device. Oct-4 and Sox2 show similar expression trend, suggesting that there may exist synergistic regulation of Sox2 and Oct-4 Role, while c-Myc may have independent function.