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目的:测定调胃舒肝丸中厚朴酚、和厚朴酚的含量。方法:HPLC法,Zorbax SB C18色谱柱(4.6 mm×150 mm,5μm),流动相甲醇-0.1%磷酸溶液(62∶38),检测波长为294 nm。结果:厚朴酚在0.145 9～0.437 8μg线性良好(r=0.999 9,n=5),和厚朴酚在0.028 5～0.085 5μg线性良好(r=0.999 6,n=5)。加样回收率厚朴酚为101.73%,RSD 0.57%(n=6)、和厚朴酚为97.27%,RSD 0.82%(n=6)。结论:本试验处理方法简单,测定结果稳定可靠,可作为调胃舒肝丸中厚朴的含量测定方法。
Objective: To determine the contents of honokiol and honokiol in Tiaowei Shugan Pill. Methods: The mobile phase was methanol-0.1% phosphoric acid solution (62:38) on a Zorbax SB C18 column (4.6 mm × 150 mm, 5 μm) with a detection wavelength of 294 nm. Results: Magnolol was linear at 0.145 9-0.437 8 μg (r = 0.999 9, n = 5) and honokiol was linear at 0.028 5-0.085 5 μg (r = 0.999 6, n = 5). The recoveries of magnolol were 101.73%, RSD 0.57% (n = 6), honokiol 97.27% and RSD 0.82% (n = 6). Conclusion: The test method is simple, the determination of the results is stable and reliable, can be used as Tiao Wei Shu Gan Wan Magnolia content determination.