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在螺旋藻生长的不同阶段分次添加无机硒(Na2SeO3)进行富硒处理。设置11个实验组,分别从第1天至第10天分次加硒,观察不同硒处理组中螺旋藻对无机硒的富集转化及其光合色素、蛋白质的变化情况。结果表明,在螺旋藻生长的第7、8、9三天分次加硒(实验组7),可获得硒产率1743.06μg/L、有机硒比率为86.85%的高富硒量螺旋藻,与对照相比,其硒含量增加150倍,生物量也明显提高(1.34g/L,对照组为1.18g/L);该实验组藻体中的硒主要分布在水溶性蛋白质中,占总硒含量的60.51%,占有机硒含量的69.67%;螺旋藻主要营养物质藻蓝蛋白(PC)、别藻蓝蛋白(APC)、类叶黄素、β-胡萝卜素含量也有不同程度的增加,表明此富硒方法是螺旋藻高值化处理的有效途径。
In different stages of the growth of Spirulina inorganic selenium (Na2SeO3) for selenium enrichment treatment. Eleven experimental groups were set up and selenium was added separately from day 1 to day 10 to observe the enrichment and transformation of inorganic selenium and the changes of photosynthetic pigments and proteins of spirulina in different selenium treatment groups. The results showed that the high selenium content Spirulina could be obtained with selenium production rate of 1743.06 μg / L and organic selenium ratio of 86.85% on the 7th, 8th, Compared with the control, the selenium content increased 150-fold, the biomass was also significantly increased (1.34g / L, the control group was 1.18g / L); selenium in the experimental group mainly distributed in water-soluble protein, accounting for 60.51% selenium and 69.67% organic selenium. The contents of PC, APC, xanthophylls and β-carotene in Spirulina also increased to some extent, This method shows that Se-enriched spirulina high value of the effective way to deal with.