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采用在十二烷基硫酸钠（ＳＤＳ）溶液中加入铜离子的方法，观察了反应温度、ＣｕＣｌ＿２及ＳＤＳ浓度等因素对主组白介素２（ｒＩＬ－２）复性的影响。确定了能够明显减少二聚体形成的复性条件，可使ｒＩＬ－２反应浓度提高到１ｍｇ／ｍｌ。结果表明，复性后形成的二聚体低于１％（ＳＤＳ－ｐＡＧＥ），ｒＩＬ－２的比活性可达到１０￣７ｕ／ｍｇ。
The effects of reaction temperature, concentrations of CuCl 2 and SDS on the renaturation of interleukin 2 (rIL-2) were observed by adding copper ions to sodium dodecyl sulfate (SDS) solution. The renaturation conditions that can significantly reduce the formation of dimers were determined, raising the rIL-2 response to 1 mg / ml. The results showed that the dimer formed after refolding was less than 1% (SDS-pAGE), and the specific activity of rIL-2 reached 10-7u / mg.