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目的观察吡格列酮对重症急性胰腺炎大鼠胰腺组织核因子-kB(NF-kB)活化的影响并探讨其意义。方法54只SD大鼠随机分成重症息性胰腺炎(SAP)组、假手术组和干预组。采用改良的Aho法制作SAP模型,采用免疫组织化学方法,动态观察3组大鼠胰腺组织中NF-kB的表达,同时观察胰腺组织病理变化。结果病理观察显示,SAP组大鼠胰腺组织有明显坏死、出血及炎症细胞聚集,而吡格列酮干预组炎症反应明显减轻。从SAP后3h起,胰腺组织中NF-kB p65即持续上调,12h与3h比较差异有统计学意义(P<0.01),呈时间依赖性;正常对照组无明显表达(P<0.01);尽管干预组NF-kB也有表达,但表达弱于SAP组。结论信号转录因子NF-kB参与了大鼠SAP的炎症反应,吡格列嗣对NF-kB的表达有抑制作用。
Objective To investigate the effect of pioglitazone on the activation of nuclear factor-kB (NF-kB) in the pancreas of rats with severe acute pancreatitis and its significance. Methods 54 Sprague-Dawley rats were randomly divided into severe SAP group, sham operation group and intervention group. SAP model was made by modified Aho method. The expression of NF-κB in pancreatic tissue of 3 groups was observed dynamically by immunohistochemical method, and pathological changes of pancreas were also observed. Results Pathological examination showed that pancreatic tissue of SAP group had obvious necrosis, hemorrhage and inflammatory cell aggregation, while the inflammatory response in pioglitazone treatment group was significantly reduced. NF-κB p65 in pancreatic tissue continuously increased from 3h after SAP, and there was significant difference between 3h and 12h (P <0.01), and it was in a time-dependent manner. There was no significant difference between normal control group and control group (P <0.01) NF-kB in the intervention group was also expressed, but weaker than the SAP group. Conclusion Signal transducer of transcription factor NF-kB is involved in inflammatory reaction of SAP in rats, and pioglitazone can inhibit the expression of NF-kB.