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目的观察左卡尼汀体外清除自由基、抗氧化活性。方法超氧阴离子(O_2~-)由黄嘌呤(X)与黄嘌呤氧化酶(XOD)反应体系产生,羟自由基(·OH)由Fenton反应体系产生,观察不同浓度下左卡尼汀口服液产生超氧阴离子自由基(O_2~-)和羟自由基(·OH)的能力;测定H_2O_2诱导大鼠红细胞氧化溶血及对EDTANa_2-Fe~(2+)-H_2O_2系统所产羟自由基诱生的大鼠红细胞膜丙二醛(MDA)含量的影响来观察左卡尼汀的生物活性。结果分别在10～100g/L及1～100g/L浓度范围内,左卡尼汀口服液产生超氧阴离子自由基和羟自由基的能力随浓度降低而增加且与稀释倍数呈良好的线性关系,直线回归方程分别为Y=14.0652+24.9066X(O_2~-)与Y=34.2673+38.0382X(·OH),相关系数(r)分别为0.9830(O_2~-)和0.9969(·OH);在10～100g/L浓度范围内,显著抑制H_2O_2诱导的大鼠红细胞氧化性溶血并显著降低大鼠红细胞膜丙二醛含量,且抑制能力与浓度呈良好的线性关系,直线回归方程分别为Y=23.5309+0.293X与Y=-0.6978+0.8971X,r分别为0.9811和0.9998。结论左卡尼汀具清除自由基及抗脂质过氧化的作用。
Objective To observe the scavenging free radical and antioxidant activity of levocarnitine in vitro. Methods The superoxide anion (O_2 ~) was produced by the reaction system of xanthine (X) and xanthine oxidase (XOD). The hydroxyl radicals (· OH) were produced by Fenton reaction system. (O 2 -) and hydroxyl radical (· OH) were measured. The oxidative hemolysis of erythrocytes induced by H 2 O 2 and the production of hydroxyl radical induced by EDTANa 2 -Fe 2 + -H 2 O 2 system Of rat erythrocyte membrane MDA (malondialdehyde) content to observe the biological activity of levocarnitine. The results showed that L-carnitine oral liquid produced superoxide anion radical and hydroxyl radical in the concentration range of 10 ~ 100g / L and 1 ~ 100g / L, respectively. The ability of L-carnitine increased with the decrease of concentration and showed a good linear relationship with the dilution , The linear regression equations were Y = 14.0652 + 24.9066X (O_2 ~ -) and Y = 34.2673 + 38.0382X (· OH) respectively, and the correlation coefficients were 0.9830 (O_2 ~) and 0.9969 10 ~ 100g / L concentration range, significantly inhibited oxidative hemolysis induced by H_2O_2 in rat erythrocytes and significantly reduced the content of MDA in rat erythrocyte membrane, and the inhibition ability showed a good linear relationship with the concentration. The linear regression equations were Y = 23.5309 + 0.293X and Y = -0.6978 + 0.8971X with r of 0.9811 and 0.9998, respectively. Conclusion L-carnitine can scavenge free radicals and prevent lipid peroxidation.