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用ＰＣＲ与Ｓｏｕｔｈｅｒｎ杂交（ＳＢＨ）方法对７８例ＨＢｓＡｇ阴性献血员进行ＨＢＶ感染检测，结果２份标本经ＰＣＲ后直接在２５４ｎｍ紫外灯下观察就可见到特异扩增带，另有１２份标本经５ｏｕｔｈｅｒｎ转膜杂交后可观察到特异扩增带，总阳性率较原来ＨＢｓＡｇ检测提高１７．９％，说明ＰＣＲ－ＳＢＨ可以区分ＨＢｓＡｇ阴性但仍然存在ＨＥＶ潜伏感染的献血员，使用ＰＣＲ－ＳＢＨ方法检测ＨＢＶ可以提高输血的安全性。
78 cases of HBsAg negative blood donors were tested for HBV infection by PCR and Southern blot hybridization (SBH). The results showed that there were 12 bands in 5 specimens The specific amplification bands were observed after transfection, the total positive rate was 17.9% higher than the original HBsAg test, indicating that PCR-SBH can distinguish HBsAg-negative but still exist latent infection of HEV blood donors, PCR-SBH detection of HBV Can improve blood transfusion safety.