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目的建立HPLC测定当归中1-(3’,4’-dihydroxycinnamoyl)-cyclopentane-2,3-diol含量的方法。方法采用Inertsil ODS-3 C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.05%三氟乙酸水溶液梯度洗脱,流速为1.0 m L·min-1,检测波长为325 nm,柱温为30℃。结果 1-(3’,4’-dihydroxycinnamoyl)-cyclopentane-2,3-diol进样量在0.023 36~1.168 0μg(r=1.000 0)内与峰面积呈良好的线性关系,平均回收率为95.33%,RSD为1.88%。结论该方法简便、准确、重复性好,可为当归药材的质量控制提供参考。
Objective To establish a HPLC method for the determination of 1- (3 ’, 4’-dihydroxycinnamoyl) -cyclopentane-2,3-diol in Angelica sinensis. Methods The mobile phase consisted of a gradient of acetonitrile-0.05% trifluoroacetic acid in acetonitrile with flow rate of 1.0 m L · min-1 on an Inertsil ODS-3 C18 column (4.6 mm × 250 mm, 5 μm) with a detection wavelength of 325 nm. The temperature is 30 ° C. Results The linear range of peak area was obtained with the injection volume of 1- (3 ’, 4’-dihydroxycinnamoyl) -cyclopentane-2,3-diol in the range of 0.023 36-1.1680 μg (r = 1.000 0) with an average recovery of 95.33 %, RSD is 1.88%. Conclusion The method is simple, accurate, reproducible and can provide reference for the quality control of Angelica sinensis.