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目的分离引起手足口病的肠道病毒,分析其基因序列特征,为手足口病的防控提供科学依据。方法从手足口病病例标本中分离病毒,提取病毒核酸并特异性扩增VPl区基因片段,进行序列测定、系统发生树构建和核苷酸同源性分析。结果从2009年河南省报告的1例手足口病病例标本中分离出1株柯萨奇B1型肠道病毒,基因序列显示其与浙江省2009年分离株同源性最高,核酸同源性达到98.4%,氨基酸同源性达100%。结论河南省手足口病病原谱中存在柯萨奇B1型病毒,并与浙江分离株属同一分支。
Objective To isolate the enterovirus which causes hand-foot-mouth disease and analyze its gene sequence characteristics to provide a scientific basis for the prevention and control of hand-foot-mouth disease. Methods The virus was isolated from the specimens of hand-foot-mouth disease, the viral nucleic acid was extracted and the VP1 gene fragment was amplified specifically for sequence analysis, phylogenetic tree construction and nucleotide homology analysis. Results One coxsackievirus type B1 enterovirus was isolated from one case of HFMD reported in Henan Province in 2009, and its sequence showed that it had the highest homology with the isolates of Zhejiang Province in 2009 and the nucleotide homology was 98.4%, amino acid homology up to 100%. Conclusion Coxsackie B1 virus exists in the pathogen spectrum of HFMD in Henan Province and belongs to the same branch with Zhejiang isolates.