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Objective To determine the eftects of zinc-detieiency and zinc-excess on hone metabolism. Methods We developed the culture model of fetal mouse limbs (16th day) cultivated in self-made rotator with continuing flow of mixed gas tor six days in vitro. The cultured limhs were examined by the techniques of 45Ca tracer and X-roentgenogruphy. Results The right limhs cultivated had longer hone length, higher bone density than the left limhs uncultivated from the same embryo: and histologically, the right limbs had active bone cell differentiation, proliferation, increased bone trabecula. clearly calcified cartilage matrix, and osteogenic tissue. Compared with the control group, the zinc-deficient group and zinc-exccss (Zn2+l2() μmol/L) group contained less osteocalcin (BGP) and 45Ca content, and lower AKP activity; whereas zine-normal (Zn2+ 45 μnol/L and Zn2+120μmol/L) groups contained more BGP and 45Ca contents, and higher AKP (alkaline phosphatase) activity. Conclusion Both zinc-deficiency and zinc-excess
Objective To determine the eftects of zinc-detieiency and zinc-excess on hone metabolism. Methods We developed the culture model of fetal mouse limbs (16th day) cultivated in self-made rotator with continuing flow of mixed gas tor six days in vitro. The cultured limhs were examined by the techniques of 45Ca tracer and X-roentgenogruphy. Results The right limhs cultivated had longer hone length, higher bone density than the left limhs uncultivated from the same embryo: and histologically, the right limbs had active bone cell differentiation, Compared with the control group, the zinc-deficient group and zinc-exccss (Zn2 + l2 () μmol / L) contained less osteocalcin (BGP) and 45Ca content , and lower AKP activity; while zine-normal (Zn2 + 45 μnol / L and Zn2 + 120 μmol / L) groups contained more BGP and 45Ca contents, and higher AKP (alkaline phosphatase) activity. cy and zinc-excess