目的　研究新城疫病毒核衣壳蛋白基因的生物学作用及其抗人喉鳞癌的作用。方法　利用含有新城疫病毒V4 株核衣壳蛋白基因cDNA的原核质粒pUVNP4 及真核质粒pcDNA3,采用基因重组技术构建了含有CMV启动子、BGHpolyA信号序列的表达新城疫病毒核衣壳蛋白的真核表达质粒。结果　酶切分析表明重组质粒中含有新城疫病毒V4 株核衣壳蛋白基因。结论　重组质粒为表达新城疫病毒核衣壳蛋白基因的真核质粒。 Objective To study the biological function of NDV nucleocapsid protein gene and its anti-human laryngeal squamous cell carcinoma. Methods The prokaryotic plasmid pUVNP4 containing the nucleocapsid protein gene of Newcastle disease virus V4 strain and the eukaryotic plasmid pcDNA3 were used to construct eukaryotic expression vector of NDV nucleocapsid protein containing CMV promoter and BGHpolyA signal sequence. Expression plasmid. Results Enzyme digestion analysis showed that the recombinant plasmids contained nucleocapsid protein gene of Newcastle disease virus V4 strain. Conclusion The recombinant plasmid is an eukaryotic plasmid expressing the NDV nucleocapsid protein gene.