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目的观察脓毒症大鼠血清谷丙转氨酶(ALT)、尿素氮(BUN)、肌酐(Cr)、肌酸激酶(CK)和乳酸脱氢酶(LDH)的变化特点并探讨其意义。方法雄性SD大鼠36只,随机分为假手术组(n=9)和盲肠穿刺结扎(CLP)组(n=27),CLP组根据术后取材时间不同分为6、12和24 h 3个亚组(n=9)。假手术组仅翻动盲肠,CLP组以CLP术制作脓毒症模型,于造模后6 h、12 h及24 h处死大鼠,取血清标本,检测血清ALT、BUN、Cr、CK和LDH水平。结果与假手术组比较,大鼠血清ALT、BUN、Cr、CK及LDH水平变化均随时间延长逐渐升高,12 h时均达到最高水平,24 h时均开始下降,但仍高于假手术组(P<0.05)。结论脓毒症大鼠血清中ALT、BUN、Cr、CK和LDH随时间依赖性改变,与脓毒症的病情严重程度密切相关,提示CLP术后大鼠肝、心、肾功能逐渐受损,12 h功能障碍最显著,12 h后器官可能已发生不可逆病变。
Objective To observe the changes of serum ALT, BUN, Cr, CK and LDH in septic rats and to explore its significance. Methods Thirty-six male Sprague-Dawley rats were randomly divided into sham operation group (n = 9) and cecal puncture (CLP) group (n = 27). CLP group was divided into 6, 12 and 24 h Subgroup (n = 9). The rats in the sham-operation group only turned the cecum. CLP rats were induced by CLP to establish sepsis model. The rats were sacrificed at 6 h, 12 h and 24 h after CLP. Serum samples were taken for detection of serum ALT, BUN, Cr, CK and LDH . Results Compared with the sham-operation group, the levels of serum ALT, BUN, Cr, CK and LDH in serum increased gradually with the increase of time, reaching the highest level at 12 h and decreasing at 24 h, but still higher than sham operation Group (P <0.05). Conclusions The changes of ALT, BUN, Cr, CK and LDH in serum of sepsis rats are time dependent and closely related to the severity of sepsis. It suggests that the function of liver, heart and kidney of rats after CLP is gradually impaired, 12 h dysfunction most significant, 12 h after the organ may have irreversible lesions.