在pH=7.40的缓冲条件下,采用荧光光谱法和紫外-可见分光光度法研究了橙黄与牛血清白蛋白(BSA)的相互作用,研究发现橙黄对BSA有较强的荧光猝灭作用,用Stern-Volmer和Lineweaver-Burk方程分别处理实验数据,得到了猝灭常数Ksv、Kq、热力学参数(ΔHθ,ΔSθ,ΔGθ)和结合位点数等,证明二者主要靠范德华作用和氢键作用,同时用同步荧光光谱法探讨了橙黄对BSA构象的影响。 Under the condition of pH = 7.40, the interaction between orange and bovine serum albumin (BSA) was studied by fluorescence spectroscopy and ultraviolet-visible spectrophotometry. It was found that orange yellow had a strong fluorescence quenching effect on BSA. The experimental data were processed by Stern-Volmer and Lineweaver-Burk equations, and the quenching constants Ksv, Kq, thermodynamic parameters (ΔHθ, ΔSθ, ΔGθ) and the number of binding sites were obtained. The effect of orange on the conformation of BSA was explored by synchronous fluorescence spectroscopy.